• Editorial
    • Genes & Diseases,Volume 6, Issue 2,2014, Pages 185-192
    • AMF siRNA treatment of keloid through inhibition signaling pathway of RhoA/ROCK1
    • YiTian1, LanJin2, WenhongZhang3, ZumengYa4, YuanCheng5, HongyunZhao6
    • 1.Department of Plastic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China;2.Department of Plastic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400011, China;3.Department of Plastic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400012, China;4.Department of Plastic Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400013, China;5.Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China;6.Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China
    Abstract
    A keloid (KD) is a benign dermal fibrotic tumor. Treatment of KDs is challenging and the recurrence rate is high; thus, there is an unmet need to explore new target sites and new treatment methods. As a tumor-associated cytokine, autocrine motility factor (AMF) can effectively stimulate the random and directional movement of cells. We first found that AMF was overexpressed in keloid fibroblasts(KFs) and the proliferation and migration of KFs were promoted by AMF stimulation. After treatment with Y-27632, RhoA kinase inhibitor, the proliferation and migration capacity of KFs declined significantly, and type I collagen protein, active RhoA and ROCK1 also were downregulated. In addition, a KD transplantation model was established under the skin of nude mice, with KD intramural injection AMF siRNA, we found that the weight of the KD was smaller than in the control group (P < 0.05), KD tissue sections stained by HE and Masson showed that fibers became loose and the blood vessels were visibly reduced. In conclusion, AMF siRNA is expected to be a novel strategy to treat KD by inhibiting signaling pathway of RhoA/ROCK1.
    Keywords
    Autocrine motility factorKeloidRhoASIRNATreatment
    Copyright © 2014 Chongqing Medical University. Published by Elsevier B.V

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